Thonotosassa is an unincorporated community located in Hillsborough County, Florida, United States. It is situated northeast of Tampa and is known for its rural character, with a mix of agricultural areas, lakes, and residential neighborhoods. The population was 15,238 at the 2020 census, up from 13,014 at the 2010 census.

John B. Sargeant, Sr. Memorial Park, 12856 U.S. Highway 301 North, is best known for boating access to the Hillsborough River. Downriver, the Hillsborough River State Canoe Trail winds through a jungle of hardwood trees, lilies and birds. Upstream, the river widens to flow through the broad hardwood swamp known as the Seventeen Runs.

The Lower Hillsborough Wilderness Preserve contains one of the largest premier contiguous recreation areas in Hillsborough County. Here one can paddle waters of the Hillsborough River amongst wading birds, turtles, alligators, lilies and cypress swamps. Explore over 60 miles of forest trails; fish from riverside boardwalks; or enjoy a relaxing picnic or cookout.